荆州地区诺如病毒流行病学及基因分型研究
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汤志明,硕士,检验技师,主要从事病原微生物分子诊断

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R181

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Epidemiological characteristics and genotyping of norovirus in Jingzhou Area
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    摘要:

    目的 了解荆州地区诺如病毒(norovirus, NoV)的流行病学及基因型特征,设计覆盖NoV基因库中变异基因组的引物和探针。方法 收集2022年1月至2023年5月来我院就诊疑似感染NoV患者粪便样本556份,使用商品化试剂盒检测粪便标本NoV核酸阳性率,并统计分析不同季节和五个年龄组阳性率差异;同时设计覆盖NoV变异基因组的引物对部分阳性标本进行基因分型。结果 被检测样品NoV核酸检出率为30.04% (167/556),其中春冬季检出率较夏秋高(χ2=20.411,P<0.01),<1岁、1~5岁、6~10岁、11~19岁、>19岁五个年龄组的阳性率有差异(χ2=17.192,P<0.01),幼童(1~5岁)来源样本NoV核酸检出率最高(39.29%,88/224);此外,抽检阳性样本均检测为NoV GII型。结论 荆州地区冬季和春季NoV流行情况严重,低龄儿童(1~5岁)感染率高,且NoV GII为主要流行基因型;且本研究设计的引物可用于NoV GI和GII的基因分型。

    Abstract:

    Objective To understand the epidemiological and genotypic characteristics of norovirus (NoV) in Jingzhou area,and to design primers and probes covering the variant genomes in the NoV gene library. Methods A total of 556 fecal samples were collected from suspected NoV patients from the First People’s Hospital of Jingzhou from January 2022 to May 2023. The positive rate of NoV nucleic acid in fecal samples was detected by commercial kits. The differences in positive rates among different seasons and five age groups were statistically analyzed. Primers covering the NoV variant genome were designed to genotype some positive specimens. Results The detection rate of NoV nucleic acid in the tested samples was 30.04% (167/556). The detection rate in spring and winter was higher than that in summer and autumn (χ2=20.411,P<0.01). There were statistical differences in the positive rates among the five age groups of <1 year, 1-5 years, 6-10 years, 11-19 years, and >19 years (χ2=17.192,P<0.01), and the positive rate in young children (1~5 years old) was the highest (39.29%, 88/224). In addition, all the positive samples were NoV GII. Conclusion The epidemic situation of NoV is serious in winter and spring in Jingzhou area, with a high infection rate in young children (1-5 years old), and NoV GII is the main prevalent genotype. The primers designed in this study can be used for genotyping of NoV GI and GII.

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  • 收稿日期:2024-12-05
  • 最后修改日期:2024-12-05
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  • 在线发布日期: 2025-01-15
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