基于基因序列分析开展广州市生食水产品副溶血性弧菌致病风险评估
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李海麟,硕士研究生,主任技师,主要从事食品安全风险监测与评估工作

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R181

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Swift quantitative risk assessment of Vibrio parahaemolyticus in aquatic products of raw food in Guangzhou by using gene sequence
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    摘要:

    目的 评估广州生食水产品副溶血性弧菌(Vibrio parahaemolyticus, VP)人群致病风险。方法 对2009—2022年广州市售生食水产品进行VP定量检测,并对30株(15株食品株、15株病患株)VP菌株进行基因测序,开展wgSNP分析,二次确定生食水产品致病性VP检出率,利用快速微生物定量风险评估软件(sQMRA),进行生食水产品VP风险评估。结果 广州市生食水产品VP检出率为7.30%(98/1 343)。病患株毒力基因TDH 检出率为 86.70%(13/15),TRH检出率为 6.67%(1/15)。15株食品株,毒力基因TDH和TRH均为阴性。wgSNP分析,食品株中有2株与病患株相似性高,为同一聚类。风险评估每年有384人因生食水产品VP感染发病。结论 广州生食水产品VP检出率高,生食水产品中检出VP毒力基因检出率低,可利用基因测序wgSNP分析开展食品致病菌风险评估,生食水产品人群VP致病风险较高。

    Abstract:

    Objective To evaluate the disease risk of Vibrio parahaemolyticus (VP) in aquatic products of raw food in Guangzhou. Methods VP detection was carried out in aquatic products of raw food sold in Guangzhou from 2009 to 2022. Gene sequence and wgSNP analysis of 30 VP strains (including 15 food strains and 15 patient strains) were performed for the detection rate of pathogenic VP. sQMRA was applied to assess VP risk of aquatic products of raw food. Results The detection rate of VP in raw aquatic products in Guangzhou was 7.30% (98/1 343). The detection rate of TDH virulence gene in patient strains was 86.70% (13/15) , and the detection rate of TRH was 6.67% (1/15). In 15 food strains, TDH and TRH were negative. The WgSNP analysis showed that 2 food strains had high similarity with the patient strains, indicating the same cluster. Risk assessment showed that the number of Vibrio parahaemolyticus infection cases caused by intaking aquatic products of raw food in Guangzhou was 384 ever year. Conclusion The detection rate of VP in aquatic products of raw food is high in Guangzhou , and the detection rate of VP virulence genes in aquatic products of raw food is low. Gene sequence and wgSNP analysis can be used for risk assessment of food pathogenic bacteria. The risk of disease of Vibrio parahaemolyticus in aquatic products of raw food is high.

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  • 收稿日期:2024-12-03
  • 最后修改日期:2024-12-03
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  • 在线发布日期: 2025-01-15
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